Coding

Part:BBa_K863030:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-18)

tvel5 laccase cDNA sequence from Trametes versicolor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 773
    Illegal BglII site found at 1205
    Illegal XhoI site found at 805
    Illegal XhoI site found at 1438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1291
    Illegal NgoMIV site found at 1444
    Illegal AgeI site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727
    Illegal SapI.rc site found at 237


Design Notes

Primers for isolation of the cDNA sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagATGTCGAGGTTTCACTCTCTTCT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTACTGGTCGCTCGGGTCGC-3'

Source

The gene sequence was originally isolated from Trametes versicolor. We got the cDNA plasmid sequence from a working group from the Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis at Ernst-Moritz-Arndt-University in Greifswald (Germany).

References